AKTA avant Chromatography System - The site saturation mutagenesis libraries were created individually for each amino acid residue by using a Quik Change mutagenesis protocol (Stratagene) with NNK as a degenerate codon (17). KTA avant is a preparative chromatography system desned for rapid and secure development of scalable chromatographic methods and processes. ÄKTA avant offers a.
AKTA FPLC System Operator's Manual - Issuu By sequence alnment using the Bio Edit software package (version 7.0.1) (13), the amino acid residues for the substrate -DAPDH were identified as Phe146, Thr171, Arg181, and His227. Issuu is a dital publishing. AKTA FPLC System Operator's Manual. Refer to the UNICORN User Manuals and to the individual component User.
KTA avant 25 Product Documentation - University of Helsinki Among all mutants, the single mutant H227V had a specific activity of 2.39 ± 0.06 U · mg-alanine with 68% yield and 99% enantiomeric excess (ee). User'sneeds. Oneormoreoptionalcomponentscanbeaddedtotheflowpath. External equipmentcanalsobeconnectedtotheinstrumentviatheI/O-boxE9.